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Description
Histone H3 (tri methyl K4) Recombinant Rabbit mAb (S-1602-21)Product Specification Host Rabbit Immunogen Synthetic Peptide Location Nucleus Accession P68431 Clone Number S 1602 21 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ChIP Reactivity Hu, Ms, Rt Positive Sample HeLa, NIH 3T3, C6 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1602-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ChIP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:5000 | Hu, Ms, Rt |
| ChIP | 1:50 |
Background
Histone H3 (tri-methyl K4) is a chromatin-associated protein that has been modified by the addition of three methyl groups to the lysine residue at position 4. This post-translational modification is typically associated with active transcription, as it creates a binding site for chromatin remodelers and transcription factors, thereby promoting an open chromatin structure that facilitates gene expression.
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