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Description
Human Cervical Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months-20° C. 1 year, see label for details |
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4.5 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
My Cart Worked Out Perfectly For Me - Update: I got a second cart
Model: Model B with Removable Tray
Delivered sooner than promised. All pieces were present. All parts were in good condition. Assembly was done by a professional who thought the product was well done and needed very few adjustments. The cart rolls smoothly, is very sturdy, is a great size, looks attractive and is perfect for my needs. I would order one of these again.
Update: I did get a second cart and I am just as happy with this second one as I have been with the first one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 8, 2022
★★★★★ 4
Notice it is not real wood!
Model: Model B with Removable Tray
Easy to put together.
The biggest complaint is it is not real wood. It looks like it is covered with contact paper!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 9, 2026
★★★★★ 5
100% recommend
Model: Model B with Removable Tray, Model: Model B with Removable Tray
Great addition to our kitchen. For something we walk by every day it was worth it to get a good quality one like this. Super well made and beautiful wood color in person.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 12, 2025
★★★★★ 5
Looks expensive. Wheels could be better.
Model: Model B with Removable Tray
This got RAVE reviews when I wheeled it into a neighbor’s Thanksgiving celebration. The guests thought it was WAY more expensive than I paid. That said, the wheels aren’t all that great; the cart got stuck going into and out of the elevator. I had to lift the cart to get it over the track. But overall, I really like it. One caution. Read the instructions carefully so that the wine racks fit. I didn’t assemble it correctly, and it became apparent when the wine racks would not fit. But I was able to quickly disassemble the necessary sections, and reassemble them quickly.
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Reviewed in the United States on November 30, 2025
★★★★★ 5
Price vs quality
Model: Model B with Removable Tray
Aside from the fact that it's beautiful ,price wise is very competitive.
We didn't want to occupy too much space in the living room so a bar cart seemed the best option.
And this one caught our eye. Easy to assemble, very sturdy and did I say beautiful ??
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 6, 2022
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