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For Your Every Summer RSVP, with Code: SUMMER15
Description
AO/EB Double Staining KitProduct Specification Usage Self prepared material: Fluorescence microscope; PBS; Cell counting board; Glass slide; Cover glass slides 1. Preparation of staining working solution Before the formal experiment, take acridine orange staining solution (reagent A), ethidium bromide staining solution (reagent B), and dilution buffer solution (reagent C), and dilute them to the required AO EB staining working solution in the ratio of A: B: C=1: 1: 8 2. Cell
Product Specification
| Usage |
Self prepared material: 1. Preparation of staining working solution |
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| Description |
Acridine orange (AO) is a heterochromatic nucleic acid binding dye with cell membrane permeability. It binds to DNA or RNA by insertion or electrostatic attraction. When combined with double stranded DNA (dsDNA), it emits green fluorescence (ex/em=502/525nm), and when combined with single stranded DNA (ssDNA) or RNA, it emits red fluorescence (ex/em=460/650nm), with a small amount of binding showing orange red fluorescence. Ethidium bromide (EB) is a polycyclic aromatic hydrocarbon fluorescent small molecule and nucleic acid insertion agent, which is chimeric into the base pairs of double stranded DNA or RNA. It has no base specificity and shows red fluorescence ((ex/em=518/605nm). EB is not cell membrane permeable
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| General Notes | 1. It is also commonly used to double stain cells with H O echst 33342, which can produce blue fluorescence after binding to dn a through the living cell membrane, and propidiu m iodide, which can only produce red fluorescence after binding to dn a through dead cells |
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| Storage Temp. | 2-8 ℃ protected from light, 6months |
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