Pay in installments of $135.38 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 18 - Jul 23
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Organotal rat normal colon organoid culture KitProduct Specification Usage 1. Primary (1) the normal colonic tissue of rats after sampling must be cleaned at 2 8 , several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it with primary culture buffer B for three times, remove impurities, remove the intestinal mucosa of normal colon tissue of rats
Product Specification
| Usage |
1. Primary (1) the normal colonic tissue of rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it with primary culture buffer B for three times, remove impurities, remove the intestinal mucosa of normal colon tissue of rats with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm3 (3) the tissue was digested with rat normal colon primary tissue digestive solution C, and then shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion process) (4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion (5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add rat normal colon organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat normal colon organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat normal colon organoid culture medium A. |
||||||||||||||||
| Synonym | Rat Colon Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
|
||||||||||||||||
| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.0 ★★★★★
Based on 24 reviews
Sort
Product Reviews
★★★★★ 5
Nice and a great value.
Color: Swift Grey, Size: 1 Pack, Style: USB Receiver
Hey, it works well. Just plug it in and you are ready to go. Only issue, minor, no light or color coding on back to indicate on or off.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Simple and efficient
Color: Swift Grey, Size: 1 Pack, Style: USB Receiver
This is a dandy little item. Easy to use straight out of the box, No fancy bells and whistles.but why should a mouse try to replicate commands that are on the laptop?
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Great.
Color: Swift Grey, Size: 1 Pack, Style: USB Receiver
Great deal. Works great.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
★★★★★ 4
It's a good working mouse but it's loud clicking and a little small.
Color: Swift Grey, Size: 1 Pack, Style: USB Receiver
I thought I was getting a quite clicking mouse but this is not it. I may have misunderstood the description or confused this mouse with another while researching. It's not a five star for me because it clicks loud and it's small and too easy to accidentally click one of the buttons when you don't want it clicked. The accidental clicking happened a lot and was becoming frustrating. As a working mouse, it does its job and it's reliable. Overall, I like the appearance of it and it feels nice and grips good. I think the price is reasonable for this product. I ended up getting a quite mouse to replace this one, but I am keeping it as a backup.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
★★★★★ 5
Very Fast
Color: Red, Size: 1 Pack, Style: USB Receiver
The Logitech M185 Wireless Mouse was exactly what I needed. This mouse works great and included a battery to get you started. No problems using it with my HP EliteBook laptop. I have to say, I was impressed with how fast I got the product. I don't think I could have driven to the store and back any faster. The service and price were worth it. Thanks again Amazon.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 7, 2026