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Description
Annexin V-APC/PI apoptosis analysis kitProduct Specification Usage 1. Self prepared instruments and reagents: 1. Reagent: PBS (abs962); EDTA free trypsin digestion (abs47014937, abs47048223) 2. Consumables and instruments: 1. 5 mL centrifuge tube (sterile and enzyme free) (abs7119), flow cytometer, fluorescence microscope, low speed centrifuge, micropipette. 2. Operation steps: For streaming operation detection 1. Cell collection (1) Suspended cells: 300 g centrifugal force, centrifugation
Product Specification
| Usage |
1. Self-prepared instruments and reagents: 1. Reagent: PBS (abs962); EDTA-free trypsin digestion (abs47014937, abs47048223) 2. Consumables and instruments: 1.5 mL centrifuge tube (sterile and enzyme-free) (abs7119), flow cytometer, fluorescence microscope, low-speed centrifuge, micropipette. 2. Operation steps: For streaming operation detection 1. Cell collection (1) Suspended cells: 300 × g centrifugal force, centrifugation for 5 min to collect; (2) Adherent cells: Adherent cells are collected by trypsin digestion without EDTA (Note: the trypsin digestion time should not be too long, otherwise it will easily cause false positives); 2. Wash the cells twice with PBS (300 × g, centrifuge for 5 min) and collect 1-5 × 105Cells; 3. Add 500μL of Binding Buffer and gently blow to form a single cell suspension; 4. Add 5μL Annexin V-APC and 5μL Propidium Iodide, and gently mix; 5. React for 5-10 min at room temperature and in the dark from light; 6. Please perform flow cytometer observation and detection within 1 hour. 7. Flow cytometry analysis (1) Detection by flow cytometry, fluorescence channel (FL4) of Annexin V-APC (Ex = 633 nm, Em = 660 nm); PI red fluorescence (Ex = 488 nm, Em ≥ 630 nm) was detected by PE channels (FL2 or FL3). (2) Fluorescence compensation adjustment: Using induction-treated apoptotic cells as a control, fluorescence compensation adjustment was performed to remove spectral overlap and set the position of cross gates. For fluorescence microscopy detection 1. Select a 24-well plate or a 48-well plate for cell culture and treatment; 2. Before staining, use a multi-well plate centrifuge 300 × g for 5 min; 3. Remove the cell culture medium and add PBS to wash once; 4. According to the number of samples, calculate and prepare the staining working solution: in a 1.5 mL EP tube, add 500μL Binding Buffer, 5μL Annexin V-APC and 5μL Propidium Iodide, and mix well (take 500μL as an example); 5. Add the staining working solution to the well plate (500μL per well for the 12-well plate, 200μL per well for the 24-well plate, 100μL per well for the 96-well plate), and incubate at room temperature in the dark for 20 minutes; 6. Observe immediately under a fluorescence microscope. |
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| Beilstein | 0 | ||||||||||||||||||||
| Synonym | Annexin V-APC/PI Apoptosis Analysis Kit | ||||||||||||||||||||
| Description |
In normal cells, phosphatidylserine (PS) is only distributed on the inside of the lipid bilayer of the cell membrane, while in the early stage of apoptosis, phosphatidylserine (PS) in the cell membrane turns from the inside to the outside of the lipid membrane. Annexin V is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36 kD. It has a high affinity for phosphatidylserine, so it can bind to the cell membrane of early apoptotic cells through the exposed phosphatidylserine on the outside of the cell. Therefore, Annexin V is one of the sensitive indicators to detect early apoptosis. Annexin V was labeled with fluorescein APC, and the labeled Annexin V was used as a fluorescent probe to detect the occurrence of apoptosis by fluorescence microscope or flow cytometry.
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| PubChem CID | 0 | ||||||||||||||||||||
| General Notes | 1. Please centrifuge and collect the solution before taking the trace reagent. 2. Annexin V-APC, Propidium Iodide (PI) is stored and used in the dark from light. 3. Propidium Iodide (PI) is toxic, please wear gloves when operating. 4. This kit is suitable for detecting living cells. When detected by flow cytometry, the number of cells should not be less than 1 × 105Units/mL, not recommended for testing tissue samples. 5. Adherent cells need to be digested with trypsin without EDTA. If digested improperly, it may cause false positives, and using a cell scraper will cause cells to adhere and form clusters, which will affect the detection. Trypsinized cells can be stored in PBS containing 2% (w/v) BSA to prevent further damage. 6. The fluorescence may be quenched after cell fixation. Please do not fix the sample. 7. Due to different types of detected cells, types of apoptosis inducers, detection instruments used, and different setting parameters, it is recommended that cells treated with apoptosis induction should be used as controls for each detection to adjust fluorescence compensation. |
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| Storage Temp. | Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months. |
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4.5 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Shark dog approved
Size: Large, Color: Broccoli, Size: Large, Color: Broccoli
My new baby shark.... I mean puppy really enjoys chewing. I can't just give her a ton of pig ears or bully sticks all day, which she would definitely love, so I'm constantly looking for well made chew toys. I'd put this right up with Kong. Very durable and nice. I also love the different textures, she seems to also love it. She can gnaw at the top and chew on the stem. She wanted it as soon as I opened it - it does smell like bacon but I haven't taken a chomp at it to be able to write a complete review!
My dog is about 23 lbs but I can see a 80 lb dog chew this, it's not small.
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Reviewed in the United States on October 8, 2025
★★★★★ 4
So cute
Size: Large, Color: Pumpkin
My French Bulldogs have really strong jaws and are aggressive chewers. These really are sturdy and last a good amount of time. They are a bit heavy and awkward for my dogs to hold onto but they do like them.
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Reviewed in the United States on April 25, 2026
★★★★★ 5
Cute toy and super tough!
Size: Small, Color: Lobster
My small dog is a serious chewer, and this lobster toy has been holding up great. Usually, he destroys toys within a day, but this one’s still going strong after a couple of weeks. The shape is perfect — easy for him to grab and chew, and the little claws keep him entertained.
The filet mignon flavor must be good because he goes right for it every time. It’s sturdy without being too hard on his teeth, and it doesn’t leave any mess or pieces behind.
If you’ve got a small but mighty chewer, this one’s definitely worth it. Cute design, lasts forever, and keeps my pup busy — total win. 🦞🐾
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Reviewed in the United States on October 26, 2025
★★★★★ 5
Excellent toy
Size: X-Large, Color: Lobster
Very durable. Dog who has destroyed every toy ever purchased loves this and it is still in great condition.
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Reviewed in the United States on April 21, 2026
★★★★★ 3
Cheese the Day with Nylabone's Power Chew Cheese Chew Toy!
Size: X-Large, Color: Cheese
As a devoted dog parent, I've always believed that our furry friends deserve the best, especially when it comes to their favorite pastime—chewing! Nylabone's Power Chew Cheese Chew Toy is a game-changer that's added an extra layer of excitement to our dog's chewing routine.
**Chew-Tastic Design:**
First things first, the cheese-inspired shape of this chew toy is a hit in our household. Our dog can't resist its quirky design, and it's become a beloved addition to his chew toy collection. It's like a fun surprise every time he reaches for it!
**Flavor Pockets for Fun:**
What truly sets this chew toy apart is its flavor pockets. We fill them with spreadable treats, turning regular chew time into an epic adventure. Our dog absolutely loves the challenge of getting every last bit of treat, and it keeps him engaged for hours.
**Dental Health Bonus:**
Beyond the fun factor, the unique textures on this toy serve a dual purpose. Not only do they provide extra sensory enjoyment for our pup, but they also help clean his teeth as he chews. It's a win-win for both entertainment and dental health.
**Built to Last:**
Now, let's talk durability. Our dog is a power chewer, and this toy has proven to be up to the challenge. Made from Nylabone's most robust material, it's stood the test of time, promoting healthy chewing habits and saving our furniture from destruction.
**Satisfaction All Around:**
This chew toy isn't just for our dog's enjoyment; it brings joy to our family too. Seeing our furry friend so engaged and happy is priceless. It satisfies his natural urge to chew while teaching him healthy chewing habits.
**A Flavorful Experience:**
Did I mention the cheese flavor? It runs throughout the toy, adding another layer of enjoyment for our pup. It's like a delicious treat that never ends!
**Perfect for Big Dogs:**
As the proud parent of an extra-large dog, we opted for the "Souper" size, designed for dogs over 50 pounds. It's the perfect size for our gentle giant.
In conclusion, Nylabone's Power Chew Cheese Chew Toy has become an integral part of our dog's life. It's more than just a chew toy; it's an adventure, a dental health solution, and hours of entertainment all rolled into one. We've embraced the mantra of "Cheese the Day" with this fantastic chew toy, and we wholeheartedly recommend it to fellow dog parents looking to add more joy and flair to their furry friend's life!
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Reviewed in the United States on October 27, 2023