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Description
Organotial Human Normal esophageal organoid Culture KitProduct Specification Description Composition: Component name Specification Human esophagus cancer organoid medium A 100mL Primary culture buffer B 250mL Human esophagus cancer primary tissue digestive juice C 30mL Organoid passage digestive juice D 30mL Tissue preservation solution E 100mL Organoid cryopreservation fluid F 20mL Organoid subculture buffer G 250mL Instructions 1, primary (1) The harvested tissues should be placed in precooled (2 8 C)
Product Specification
| Description |
Composition:
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| Instructions |
1, primary (1) The harvested tissues should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. (2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. (3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm3in volume using ophthalmic scissors or scalpel. (4) The tissues were digested with normal human esophageal primary tissue digestion solution C and shaken at 37℃ for 20-25min (the digestion was observed at any time during the digestion). (5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. (6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. (7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. (8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). (9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to become gelation, and human normal esophageal organoid medium A was added for culture (return to room temperature). 2, Organoid subculture (1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. (4) After the organoid was collected, 25uL of Matrigel was added to the 24-well cell culture plate, and 500uL of human normal esophageal organoid medium A was added to the incubator for 10-15min. 3. Organoids were frozen (1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4℃ organoid passage culture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. (4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. (5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. 4, organoid resuscitation (1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. (2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. (3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. (4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. (5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of human normal esophageal organoid medium A was added. |
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| Storage Temp. | Stored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details. |
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4.0 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Great for Tough chewers!
Size: Large, Color: Dinos Frills (Red)
My dog is a tough chewer and absolutely loves this toy! He was able to get a little stuffing out of the leg so far and it’s been about a week. This might sound like a short amount of time but it usually takes him under 15 mins to unstuff a large toy even if they are advertised for “tough chewers!” This is a very large size toy and would be good for any larger breed. He carries it around the house and constantly keeps his Dino with him. Since Ive found GoDog toys I’ve only been purchasing them because of their durability. I’ve tried Kong and Bark toys previously and they just do hold up long and are usually more expensive. This toy is a great value and dogs love them!
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Reviewed in the United States on October 20, 2025
★★★★★ 5
Super cool strong dog toy!
Size: Large, Color: Dinos Frills (Red)
Super cool strong dog toy!
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Reviewed in the United States on May 12, 2026
★★★★★ 4
LOVE this brand. Have many of their styles. This is basically an unstuffed toy.
Size: Large, Color: Dinos Frills (Gray)
I have about 10 -12 different styles of the GoDog brand toys. It is like Xmas when my Malinois dogs get a new GoDog toy. Have loved this brand for many years and have some toys which have lasted 5-6 years -- minor "surgeries" stitch they back together -- until I retired them. They have never just worn out. Just to be clear:
THESE TOYS ARE NOT CHEW PROOF. NO STUFFED TOY IS CHEW PROOF.
However, this brand of stuffed toy is more chew proof than other brands. But if you are looking for a chew proof stuffed toy be advised -- IT DOES NOT EXIST!! No fabric is chew proof. Even if they invented such a fabric I would not want to ruin my dog's teeth by letting them try to chew through it. Protecting one's dog's teeth should be the highest priority.
If a dog can chew through a PLASTIC KENNEL and even METAL WIRE KENNELS (yes, it happens) then they can easily chew through a puffy, fluffy stuffed toy. The "Chew Guard Technology" language may be misleading to some customers who are disappointed in the brand. I defend the brand and have used it for many years. No, I don't get paid or get free toys.
Since I have had many styles within this brand and even have the pink version of this style I thought I knew what I was getting. This is basically a toy without stuffing which some people prefer. For whatever reasons my dogs prefer toys that are stuffed. Really stuffed with a firm density that they can sink their teeth into. So this toy will be OK. I can tell it will not be one of their beloved toys like some of the brand's other styles such as the purple brontosaurus. My fault for not reading more closely and assuming the grey version would be exactly like the pink version which is fully stuffed. If they get bored with these two I keep some of the Chew Guard toys stock piled.
BTW one can wash and dry these toys. Yes, eventually the squeakers suck up some water and die but one can replace the squeakers. Also on Amazon. So I am willing to replace the squeakers a few times in the life time of one of these toys to be able to wash and dry them occasionally. Usually takes a couple drying cycles to get the heavily stuffed toys dried completely.
If you have an aggressive chewer you may wish to look into Goughnuts dog toys or other hard rubber toys that are designed for aggressive chewers. Even then, with a determined dog, the Goughnuts toys can be chewed through. I think they offer a replacement guarantee. The other brand which has some toys of hard rubber is West Paw. An excellent brand that also offers a one time replacement. Bully Sticks are another option for chewers.
PLEASE, PLEASE, PLEASE stay away from the hard plastic chew toys like the Nyla Bones. Hard plastic toys will destroy your dog's teeth over time. I learned the hard way (no pun intended) and ruined one of my dog's teeth. Vet asked if I allowed my dog to chew ROCKS !!!!!! Every tooth was cracked or fractured. Not wishing to pull teeth I had to pay for some very expensive root canals and caps. Vet's advise: If you can't dent the toy or chew with your finger nail DO NOT offer to your dog. Good advise to follow. Even some of the Bully Sticks (Costco Brand) are so hard they can chip or fracture teeth.
Best for you and your dog.
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Reviewed in the United States on September 1, 2019
★★★★★ 5
Well made, minor issue was quickly resolved
Size: Large, Color: Dinos Bruto (Purple)
I purchased the Dino Bruto Dog toy for my labradoodle who is quite fond of squeaky, squawky stuffed toys and oddly enough has shown a preference for purple. I opened the package with my hound standing by waiting for his gift. I detached the tags and attempted to squeak the toy. It seemed difficult to squeak or honk. I tossed the purple Dino Bruto to my dog who excitedly chomped to squeak. The toy squawked a couple times and then just quit. My poor hound was staring up at me with that "what's up with this" look on his face. I tracked down the website for the GoDog toys, not going through the Amazon return process. I called the good people at GoDog and stated simply that my dog's favorite part of the toy failed quite quickly and I was wondering about guarantees. I was told to go to the website and file a replacement request. The replacement request required that I include a photo which I did as directed. I received an reply email within a couple hours which stated that a new toy would be sent out shortly.
I am happy to report that we received a replacement toy, not the purple Dino, but a colorful wood duck. The squawker device works well and is still performing like new more than a month later.
It appears that the squeaker is not those little plastic squeakers but the bigger heavy plastic bag, bladder, with what I visualize as a duck call sort of sound device. I am guessing because I haven't seen the inside of the GoDog toys. I have seen the guts of other stuffed toys which use this type of noise device. While our dog seems to enjoy the sound of the plastic bag, bladder set-up squawkers, they seem to fail quickly and I tend to avoid them because of this. The toys using this type of squawker that I have seen, use what looks like a cheap lightweight plastic bag which is not securely attached to the "voice box".
Since the GoDog replacement toy is holding up so well, I am guessing GoDog uses a heavier grade plastic bladder coupled with reinforced construction. The purple Dino Bruto toy still looks new even after multiple times of our hound grabbing the purple toy, shaking it at us with his invite to give chase or play keep away. Since the squawker failed so quickly with the new toy, I figure it was just a defective item which does not reflect the general quality of toys from GoDog.
One evening our neighbor was visiting while his pitbull was playing in our yard with our dog. Our patio door was open so our dog ran into the house and grabbed his purple Dino Bruto toy. He is a bit of a show-off with new toys. Our dog then went racing back into the yard, shaking his GoDog toy at the pitbull. A quick game of tug-of-war ensued. Since this is a new toy, our neighbor leaped into the game and took the toy away from the dogs. In that short time of tugging between the dogs, the toy held together and did not even pull apart at any of the seams. There were no puncture marks either.
This is a game our dog has initiated before with the neighbor pitbull. Normally, the stuffed toys are ripped apart almost immediately. I was surprised and impressed with the quality of the sewing which appears to employ some sort of reinforced construction or heavier material.
I plan to make more purchases from GoDog toys. The toys are a little more expensive than some stuffed dogs toys but they hold up so well I figure they are worth a few more dollars. They are great toys that hold up well and the company provides quick responses to inquiries. I consider this a winning combination.
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Reviewed in the United States on June 15, 2013
★★★★★ 5
Good quality
Size: Large, Color: Dinos Frills (Gray)
we’ve had many godog toys over the years and although my dog eventually rips through them I do find that they last longer than other cheap toys. Overall very well made!
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Reviewed in the United States on May 11, 2026
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