SKU: 23223567299

Ready to use immunohistochemical secondary antibody kit (anti mouse)

Sale price$112.50 Regular price$125.00
Save 10%

Pay in installments of $31.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 18 - Jul 23

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Ready to use immunohistochemical secondary antibody kit (anti mouse)Product Specification Description packaging: 5ml (can be used for 50 pieces) applicable: primary antibody is from mouse chromogenic substrate: dab (benzidine) reagent composition: component name specification quantity Endogenous peroxidase blocker 5ml 1 HRP enzyme labeled anti mouse secondary antibody polymer 5ml 1 DAB substrate (20) 0. 25ml 1 DAB diluent 5ml 1 Hematoxylin 5ml 1 reagents not provided but required: primary antibody (from mouse), 5%

Product Specification

Description

packaging: 5ml (can be used for 50 pieces)

applicable: primary antibody is from mouse

chromogenic substrate: dab (benzidine)

reagent composition:

component name specification quantity
Endogenous peroxidase blocker 5ml 1
HRP enzyme labeled anti mouse secondary antibody polymer 5ml 1
DAB substrate (20×) 0.25ml 1
DAB diluent 5ml 1
Hematoxylin 5ml 1

reagents not provided but required: primary antibody (from mouse), 5% goat serum (abs933), pbst

this kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time

storage and validity period

2-8° C save. Each component can be stored for at least 12 months



operation steps:

note: all the steps are operated at room temperature. The reagent can be directly titrated in a drop bottle or with a pipette. One drop =30-40ul

1Dewaxed and hydrated tissue sections

2Wash 2-3 times in PBST for 5 minutes

3According to the special requirements of the applied primary antibody, the tissue sections were pretreated (antigen retrieval)

4Wash 2-3 times in PBST for 5 minutes

5Add 100ul endogenous peroxidase blocking agent was incubated for 10 min to block endogenous peroxidase to reduce nonspecific background staining

6Wash 2-3 times in PBST for 5 minutes

7Add 100ul of 5% to 10% normal goat serum and incubate for 20 minutes at room temperature. If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted

8Wash 2-3 times in PBST for 5 minutes

9Add primary antibody and incubate at room temperature or 37 ℃ for 30 min

10Wash 2-3 times in PBST for 5 minutes

11Add 100ul HRP enzyme labeled anti mouse & Rabbit secondary antibody polymer was incubated for 30 min   Note: this liquid is sensitive to light, and keep away from light

12Wash 2-3 times in PBST for 5 minutes

13Use freshly prepared dab chromogenic solution (Dilute with dab Dilution DAB substrate , ready to use) , incubate at room temperature for 5 minutes (the time can be adjusted according to the color development), and rinse the slide with tap water

14Add 100ulhematoxylin , incubate for 3 minutes (the time can be appropriately adjusted according to the color development), and rinse with tap water to return to blue

15Dehydrated, transparent, sealed

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 23223567299

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.0 ★★★★★
Based on 15 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
J
Verified Purchase
Jess
Birmingham, US
★★★★★ 5
Great for kids!
Color: White
I bought this stylus for the kids to use with their iPads, and it definitely gets the job done. It connects easily, charges fast, and works smoothly for drawing, writing, and playing around on apps. The palm rejection is a big plus—it makes it a lot easier for them to use without messing up what they’re doing. It’s also pretty responsive, which I wasn’t necessarily expecting at this price. Overall, it served its purpose well. If you’re looking for something affordable and functional for kids (or even light everyday use), this is a solid option.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 22, 2026
T
Verified Purchase
Thomas
San Leandro, US
★★★★★ 5
Rock-Solid Apple Pencil Replacement for iPad Mini
Color: White
Despite the box arriving pretty beat up inside the Amazon packaging, the pen itself was in perfect condition and it’s been outstanding. It works flawlessly with my iPad Mini (Gen 7, A17). The physical on/off button is a standout feature for me; the tactile click is far more satisfying and reliable than the touch-sensitive controls found on other pens. I primarily use this stylus while flying, taking notes in the air, and it performs exactly how I need it to. The magnetic attachment is especially impressive because it’s strong enough to add real security inside my iPad case. Even in rough air, I don’t have to worry about it getting knocked loose. I previously used a pencil from another brand that worked fine overall, but the weak (or nonexistent) magnetic hold was a dealbreaker. This one solves that problem completely and when it came time to buying a new pencil because I lost the old one, this was my choice. If you’re looking for a dependable, well-built Apple Pencil replacement with a solid magnetic connection and a satisfying physical control, this is an easy recommendation. You also CANNOT go wrong with the price!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
D
Verified Purchase
DLE
Dallas, US
★★★★★ 5
Perfect stylus for my iPad.
Color: White
This stylus works great! It was easy to set up, and is perfect for my needs. The pen is the perfect weight and seems to be made of good material. I anticipate it will last a long time. The pen came charged and I still haven’t charged it. If you like using a stylus on an iPad, this one feels just like using a pencil. I like to write, so this has been a great choice.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 9, 2026
T
Verified Purchase
Taylor
Birmingham, US
★★★★★ 5
Great product!
Color: White
I have now purchased two of these (I lost the first one) and they are absolutely a worthwhile purchase. Nicely packaged, easy set up, works great! Battery life is great and the pencil charged fairly quickly as well. Size of the pencil is wonderful, easy to hold and grip. Quality is great as well. 5 stars from me, hopefully I don’t lose this one! 🤣
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2026
Q
Verified Purchase
Quynn Campbell
Louisville, US
★★★★★ 5
Works amazingly as intended, good value
Color: White, Color: White
Writes perfectly on my ipad. Worked immediately on my Bluetooth, didn’t have to connect. Holds charge very well from what I can tell. I finished a 10 hour long drawing process without it dying. I’ll update if anything changes. Comes with extra nibs.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 9, 2026

recommand products